Supplementary Materials

Supplementary Materials for:

Activation of a Bacterial Virulence Protein by the GTPase RhoA

Matthias Christen, Lisette H. Coye, Jill S. Hontz, Doris L. LaRock, Richard A. Pfuetzner, Megha, Samuel I. Miller*

*To whom correspondence should be addressed. E-mail: millersi{at}u.washington.edu

This PDF file includes:

  • Fig. S1. Assessment of the interaction of SseJ with various GTPγS-loaded GTPases and their ability to stimulate its lipase activity.
  • Fig. S2. Cotransfection of HeLa cells with plasmids encoding CA RhoA and SseJ results in the accumulation of cholesterol and neutral lipids in the enlarged endosomal compartment.
  • Fig. S3. The expression of SseJ3x or RhoA alone does not induce the formation of BODIPY-labeled cholesterol esters.
  • Table S1. Descriptions of the strains of S. typhimurium and the plasmids used in this study.
  • References

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M. Christen, L. H. Coye, J. S. Hontz, D. L. LaRock, R. A. Pfuetzner, Megha, S. I. Miller, Activation of a Bacterial Virulence Protein by the GTPase RhoA. Sci. Signal. 2, ra71 (2009).

© 2009 American Association for the Advancement of Science