Supplementary Materials

Supplementary Materials for:

RIAM Regulates the Cytoskeletal Distribution and Activation of PLC-γ

Nikolaos Patsoukis, Esther M. Lafuente, Paul Meraner, Jin sub Kim, David Dombkowski, Lequn Li, Vassiliki A. Boussiotis*

*To whom correspondence should be addressed. E-mail: vboussio{at}

This PDF file includes:

  • Fig. S1. Colocalization of RIAM with ZAP-70 and F-actin at the IS.
  • Fig. S2. Reduced abundance of RIAM in RIAM-KD Jurkat T cell lines.
  • Fig. S3. Effects of knockdown of RIAM on Ca2+ mobilization.
  • Fig. S4. The interaction between PLC-γ1 and RIAM requires the C-terminal PRR of RIAM.
  • Fig. S5. Reconstitution of RIAM-KD cells with RIAM-D1 does not restore their ability to activate MEK, ERK, or JNK.
  • Fig. S6. Negative controls used in detecting the colocalization of phosphorylated PLC-γ
  • Fig. S7. Negative controls used in detecting colocalization of PIP2 with the actin cytoskeleton.
  • Fig. S8. RIAM and LAT associate with separate pools of PLC-γ
  • Fig. S9. Knockdown of RIAM does not affect the abundance of pLAT.
  • Fig. S10. Analysis of the purity of cytoskeletal fractions.

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Citation: N. Patsoukis, E. M. Lafuente, P. Meraner, J. s. Kim, D. Dombkowski, L. Li, V. A. Boussiotis, RIAM regulates the cytoskeletal distribution and activation of PLC-γ1 in T cells. Sci. Signal. 2, ra79 (2009).

© 2009 American Association for the Advancement of Science