Supplementary Materials

Supplementary Materials for:

Noncoding RNA Gas5 Is a Growth Arrest– and Starvation-Associated Repressor of the Glucocorticoid Receptor

Tomoshige Kino,* Darrell E. Hurt, Takamasa Ichijo, Nancy Nader, George P. Chrousos

*To whom correspondence should be addressed. E-mail: kinot{at}mail.nih.gov

This PDF file includes:

  • Fig. S1. Translation into protein is not required for Gas5 to repress GR-induced transcriptional activity in HeLa cells.
  • Fig. S2. Gas5 suppresses association of GR to cIAP2 GREs.
  • Fig. S3. Gas5 expression correlates with suppression of GR-induced cIAP2 mRNA expression, whereas that of U75 does not.
  • Fig. S4. Cells have similar amounts of the Gas5 fragments and cells expressing these fragments have similar amounts of GR.
  • Fig. S5. Sequences of wild-type Gas5 and Gas5 mutants used in the analyses.
  • Fig. S6. Repression of Gas5 accumulation and activity with siRNA in serum-starved cells.
  • Table S1. Copy numbers of Gas5 in HeLa cells cultured in the presence and absence of dexamethasone.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 198 KB


Citation: T. Kino, D. E. Hurt, T. Ichijo, N. Nader, G. P. Chrousos, Noncoding RNA Gas5 Is a Growth Arrest– and Starvation-Associated Repressor of the Glucocorticoid Receptor. Sci. Signal. 3, ra8 (2010).

© 2010 American Association for the Advancement of Science