Supplementary Materials

Supplementary Materials for:

Regulation of Zap70 Expression During Thymocyte Development Enables Temporal Separation of CD4 and CD8 Repertoire Selection at Different Signaling Thresholds

Manoj Saini, Charles Sinclair, Daniel Marshall, Mauro Tolaini, Shimon Sakaguchi, Benedict Seddon*

*To whom correspondence should be addressed. E-mail: bseddon{at}nimr.mrc.ac.uk

This PDF file includes:

  • Fig. S1. Rapid induction of Zap70 expression after the administration of dox to TetZap70 mice.
  • Fig. S2. Abundance of co-receptors on DP thymocytes after induced production of Zap70 protein.
  • Fig. S3. Rapid induction of ThPOK production in CD4+ SP cells from dox-fed TetZap70 mice.
  • Fig. S4. Cell populations of peripheral lymph nodes in dox-fed TetZap70 mice.
  • Fig. S5. Intrathymic development of Foxp3+ Tregs from TetZap70 thymocytes.
  • Fig. S6. NK1.1+ NK T cells fail to develop in TetZap70 mice fed a dox-containing diet.
  • Fig. S7. TCR and CD5 define developmentally distinct populations of DP thymocytes.
  • Fig. S8. The abundance of Zap70, Lck, SLP-76, LAT, and ERK in various thymocyte subsets from wild-type and TetZap70 mice.
  • Fig. S9. INDO dye loading, Ca2+ flux responses to ionomycin, and generation of pERK in response to phorbol ester by wild-type and TetZap70 thymocytes.
  • Fig. S10. The increased abundance of Zap70 protein in thymocytes from SKG mice.
  • References.

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Citation: M. Saini, C. Sinclair, D. Marshall, M. Tolaini, S. Sakaguchi, B. Seddon, Regulation of Zap70 Expression During Thymocyte Development Enables Temporal Separation of CD4 and CD8 Repertoire Selection at Different Signaling Thresholds. Sci. Signal. 3, ra23 (2010).

© 2010 American Association for the Advancement of Science