Supplementary Materials

Supplementary Materials for:

Negative Feedback in Noncanonical NF-κB Signaling Modulates NIK

Stability Through IKKα-Mediated Phosphorylation

Bahram Razani, Brian Zarnegar, A. Jimmy Ytterberg, Travis Shiba, Paul W. Dempsey, Carl F. Ware, Joseph A. Loo, Genhong Cheng*

*To whom correspondence should be addressed. E-mail: gcheng{at}mednet.ucla.edu

This PDF file includes:

  • Fig. S1. Ectopic expression of IKKα results in destabilization of ectopically expressed NIK.
  • Fig. S2. Truncated mutants of NIK continue to interact with IKKα.

    Fig. S3. IKKα phosphorylates Ser809, Ser812, and Ser815 of NIK.
  • Fig. S4. NIK-3SA does not affect the interaction between wild-type NIK and TRAF3 or its degradation by the TRAF-cIAP complex.
  • Fig. S5. Ubiquitination of NIK is not enhanced by the presence of IKKα in HEK 293T cells.

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Citation: B. Razani, B. Zarnegar, A. J. Ytterberg, T. Shiba, P. W. Dempsey, C. F. Ware, J. A. Loo, G. Cheng, Negative Feedback in Noncanonical NF-κB Signaling Modulates NIK Stability Through IKKα-Mediated Phosphorylation. Sci. Signal. 3, ra41 (2010).

© 2010 American Association for the Advancement of Science