Supplementary Materials

Supplementary Materials for:

EGF Decreases the Abundance of MicroRNAs That Restrain Oncogenic Transcription Factors

Roi Avraham, Aldema Sas-Chen, Ohad Manor, Israel Steinfeld, Reut Shalgi, Gabi Tarcic, Noa Bossel, Amit Zeisel, Ido Amit, Yaara Zwang, Espen Enerly, Hege G. Russnes, Francesca Biagioni, Marcella Mottolese, Sabrina Strano, Giovanni Blandino, Anne-Lise Børresen-Dale, Yitzhak Pilpel, Zohar Yakhini, Eran Segal, Yosef Yarden*

*To whom correspondence should be addressed. E-mail: yosef.yarden{at}weizmann.ac.il

This PDF file includes:

  • Supplementary Text: Statistical Analysis, including analyses of patients.
  • Fig. S1. Dynamic alterations of microRNA abundance after stimulation with EGF or serum.
  • Fig. S2. EGF-regulated microRNAs associate with specific subsets of two tumor types.
  • Fig. S3. Effects of ID-miR overexpression on EGF-induced migration and on expression of putative target IEGs.
  • Fig. S4. Regulation of EGR1 by ID-miRs.
  • Fig. S5. Putative functions of predicted mRNA targets of EGF- and serum-regulated microRNAs.
  • Fig. S6. Examples of network motifs, including EGF-regulated microRNAs and co-occurring transcription factors (TFs).
  • Table S1. MicroRNA abundance analysis in MCF10A and HeLa cells treated with EGF or serum for the indicated time intervals.
  • Table S2. Clinical characteristics of breast cancer patients whose tumors were used for the analysis of microRNA abundance in EGFR/HER2-amplified vs. nonamplified tumors.
  • Table S3. Clinical characteristics of breast cancer patients whose tumors were used for the analysis of microRNA abundance in tumor vs. peritumor samples.
  • Table S4. Analysis of predicted targets of individual microRNAs.
  • Table S5. mRNAs induced by Dicer knockdown in starved MCF10A cells.
  • Table S6. Analysis of correlation between abundance of ID-miRs and abundance of predicted target IEGs in breast cancer patients.
  • Table S7. Analysis of correlation between microRNA abundance and that of predicted target genes.
  • Table S8. Analysis of enrichment of microRNA-TF pairs.
  • Table S9. Primers used for cloning and real-time PCR.
  • References

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Citation: R. Avraham, A. Sas-Chen, O. Manor, I. Steinfeld, R. Shalgi, G. Tarcic, N. Bossel, A. Zeisel, I. Amit, Y. Zwang, E. Enerly, H. G. Russnes, F. Biagioni, M. Mottolese, S. Strano, G. Blandino, A.-L. Børresen-Dale, Y. Pilpel, Z. Yakhini, E. Segal, Y. Yarden, EGF Decreases the Abundance of MicroRNAs That Restrain Oncogenic Transcription Factors. Sci. Signal. 3, ra43 (2010).

© 2010 American Association for the Advancement of Science