Supplementary Materials

Supplementary Materials for:

Nodal Signaling Recruits the Histone Demethylase Jmjd3 to Counteract Polycomb-Mediated Repression at Target Genes

Øyvind Dahle, Amit Kumar, Michael R. Kuehn*

*To whom correspondence should be addressed. E-mail: mkuehn{at}

This PDF file includes:

  • Fig. S1. Quantification of the abundance of Nodal mRNA in WT ES cells and in B16
    melanoma cells.
  • Fig. S2. ChIP analysis of Smads2/3 at the PEE and ASE regions of the Nodal locus.
  • Fig. S3. The abundance of total histone H3 at the Nodal and Brachyury loci.
  • Fig. S4. Changes in Nodal expression and chromatin status after 24 hours of
    signaling inhibition.
  • Fig. S5. Expression of Nanog and Oct4 after knockdown of Jmjd3.
  • Table S1. Primers used for RT-PCR analysis.
  • Table S2. Primers used for ChIP analysis.

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Citation: Ø. Dahle, A. Kumar, M. R. Kuehn, Nodal signaling recruits the histone demethylase Jmjd3 to counteract Polycomb-mediated repression at target genes. Sci. Signal. 3, ra48 (2010).

© 2010 American Association for the Advancement of Science