Supplementary Materials

Supplementary Materials for:

Increased Abundance of Opioid Receptor Heteromers After Chronic Morphine Administration

Achla Gupta, Jan Mulder, Ivone Gomes, Raphael Rozenfeld, Ittai Bushlin, Edmund Ong, Maribel Lim, Emeline Maillet, Mats Junek, Catherine M. Cahill, Tibor Harkany, Lakshmi A. Devi*

*To whom correspondence should be addressed. E-mail: lakshmi.devi{at}

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation and characterization of μ-δ heteromer–selective antibodies.
  • Fig. S2. Subcellular distribution and colocalization of μ and δ opioid receptors.
  • Fig. S3. Chronic morphine treatment induces increased μ-δ heteromer

    immunoreactivity in the brainstem.
  • Fig. S4. Chronic morphine treatment increases μ-δ heteromer abundance.
  • Fig. S5. μ-δ heteromer–selective antibodies block heteromer-mediated binding and

  • Table S1. Characterization of select hybridoma clones.
  • Table S2. Heteromer-selective antibody recognition using chimeric μ-δ constructs.
  • References

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Citation: A. Gupta, J. Mulder, I. Gomes, R. Rozenfeld, I. Bushlin, E. Ong, M. Lim, E. Maillet,

M. Junek, C. M. Cahill, T. Harkany, L. A. Devi, Increased Abundance of Opioid Receptor

Heteromers After Chronic Morphine Administration. Sci. Signal.3, ra54 (2010).

© 2010 American Association for the Advancement of Science