Supplementary Materials

Supplementary Materials for:

Autocrine Purinergic Receptor Signaling Is Essential for Macrophage

Chemotaxis

Moritz Kronlage, Jian Song, Lydia Sorokin, Katrin Isfort, Tanja Schwerdtle, Jens Leipziger, Bernard Robaye, Pamela B. Conley, Hee-Cheol Kim, Sarah Sargin, Peter Schön, Albrecht Schwab, Peter J. Hanley*

*To whom correspondence should be addressed. E-mail: hanley{at}uni-muenster.de

This PDF file includes:

  • Fig. S1. Reversed-phase HPLC analysis of ATP degradation by potato apyrase.
  • Fig. S2. Inhibition of the adenosine feedback loop with 8-SPT.
  • Fig. S3. Chemotaxis is impaired by blocking ecto-ATPase in P2ry2-deficient

    macrophages.
  • Fig. S4. Chemotaxis is not impaired in Panx1-deficient macrophages.
  • Fig. S5. The P2Y12 receptor antagonist AR-C69931MX blocks ADP-β-S–induced

    lamellipodial membrane protrusions.
  • Descriptions of movies S1 to S13.

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Technical Details

Format: Adobe Acrobat PDF

Size: 348 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. Wild-type macrophages in a C5a chemotactic gradient (.mov format).

    [Download Movie S1]
  • Movie S2. Wild-type macrophages in a C5a chemotactic gradient with apyrase (40 U/ml) (.mov format).

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  • Movie S3. P2ry2–/– macrophages in a C5a chemotactic gradient (.mov format).

    [Movie Movie S3]
  • Movie S4. P2ry12–/– macrophages in a C5a chemotactic gradient (.mov format).

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  • Movie S5. P2ry2–/– macrophages in a C5a chemotactic gradient with AR-C69931MX (10 μM) (.mov format).

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  • Movie S6. P2ry2–/– macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).

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  • Movie S7. P2ry12–/– macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).

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  • Movie S8. Wild-type macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).

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  • Movie S9. P2ry2–/– macrophages in a C5a chemotactic gradient with "cocktail block" (.mov format).

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  • Movie S10. P2ry2–/– macrophages in a C5a chemotactic gradient with "triple block" (.mov format).

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  • Movie S11. Panx1–/– macrophages in a C5a chemotactic gradient (.mov format).

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  • Movie S12. ATP-induced lamellipodial membrane protrusions (.mov format).

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  • Movie S13. Lack of ATP-induced lamellipodial formation in P2ry2–/– macrophages (.mov format).

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Citation: M. Kronlage, J. Song, L. Sorokin, K. Isfort, T. Schwerdtle, J. Leipziger, B. Robaye, P. B. Conley, H.-C. Kim, S. Sargin, P. Schön, A. Schwab, P. J. Hanley, Autocrine Purinergic Receptor Signaling Is Essential for Macrophage Chemotaxis. Sci. Signal.3, ra55 (2010).

© 2010 American Association for the Advancement of Science