Supplementary Materials

Supplementary Materials for:

Arginine Usage in Mycobacteria-Infected Macrophages Depends on Autocrine-Paracrine Cytokine Signaling

Joseph E. Qualls, Geoffrey Neale, Amber M. Smith, Mi-Sun Koo, Ashley A. DeFreitas, Huiyuan Zhang, Gilla Kaplan, Stephanie S. Watowich, Peter J. Murray*

*To whom correspondence should be addressed. E-mail: peter.murray{at}

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The expression of Arg1 and C/EBPβ during infection with BCG is dependent on MyD88.
  • Fig. S2. Principal components analysis (PCA) of the Affymetrix transcript array.
  • Fig. S3. C/EBPβ is partially required for the expression of Arg1 after stimulation with supernatant from BCG-infected cells.
  • Fig. S4. BCG-induced expression of Arg1 is independent of the alternative activation of macrophages.
  • Fig. S5. IL-6, IL-10, and G-CSF are produced by wild-type, but not Myd88–/–, macrophages after infection with BCG.
  • Fig. S6. Expression of Arg1 is induced by recombinant IL-6, IL-10, and G-CSF.
  • Fig. S7. The expression of Arg1 is intact in BMDMs from Il6–/– mice after infection with BCG.

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Citation: J. E. Qualls, G. Neale, A. M. Smith, M.-S. Koo, A. A. DeFreitas, H. Zhang, G. Kaplan, S. S. Watowich, P. J. Murray, Arginine usage in mycobacteria-infected macrophages depends on autocrine-paracrine cytokine signaling. Sci. Signal. 3, ra62 (2010).

© 2010 American Association for the Advancement of Science