Supplementary Materials

Supplementary Materials for:

Self-Induced Patched Receptor Down-Regulation Modulates Cell Sensitivity to the Hedgehog Morphogen Gradient

Andreu Casali*

*To whom correspondence should be addressed. E-mail: actbmc{at}ibmb.csic.es

This PDF file includes:

  • Fig. S1. In situ hybridization to detect GFP-positive transcripts.
  • Fig. S2. PtcΔ2-GFP (from the L>PtcΔ2-GFP transgene) is down-regulated in leg and haltere discs.
  • Fig. S3. PtcLDL is endocytosed and sequesters Hh, but does not repress Smo.
  • Fig. S4. Overexpression of Dispatched (Disp) does not induce degradation of Ptc-GFP (from the L>Ptc-GFP transgene).
  • Fig. S5. PtcAAAA-GFP regulates Smo activity in the absence of endogenous Ptc.
  • Fig. S6. The GFP tag does not affect the ability of PtcΔ2 to interact with endogenous Ptc.

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Citation: A. Casali, Self-Induced Patched Receptor Down-Regulation Modulates Cell Sensitivity to the Hedgehog Morphogen Gradient. Sci. Signal. 3, ra63 (2010).

© 2010 American Association for the Advancement of Science