Supplementary Materials

Supplementary Materials for:

Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis

Ping Che,* John D. Bussell, Wenxu Zhou, Gonzalo M. Estavillo, Barry J. Pogson, Steven M. Smith*

*To whom correspondence should be addressed. E-mail: ssmith{at}cyllene.uwa.edu.au (S.M.S.); pche{at}cyllene.uwa.edu.au (P.C.)

This PDF file includes:

  • Fig. S1. A diagram of regulated intramembrane proteolysis.
  • Fig. S2. Localization of S1P, S2P, and bZIP28.
  • Fig. S3. Characterization of s2p mutant.
  • Fig. S4. Structures of full-length and truncated bZIPs.
  • Fig. S5. S2P-RIP does not affect BR biosynthesis gene expression.
  • Fig. S6. BES1 does not induce chaperone gene expression in wild-type or s2p backgrounds.
  • Fig. S7. Possible interactions of stress-induced S2P-RIP with BR signaling pathway.
  • Table S3. Oligonucleotides used for PCR, RT-PCR, and qRT-PCR.
  • Table S4. Oligonucleotides used for plasmid constructs.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Complementation of bri1-5 down-regulated genes by bZIPΔC (Microsoft Excel format).
  • Table S2. Complementation of bri1-5 up-regulated genes by bZIPΔCs (Microsoft Excel format).

Citation: P. Che, J. D. Bussell, W. Zhou, G. M. Estavillo, B. J. Pogson, S. M. Smith, Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis. Sci. Signal. 3, ra69 (2010).

© 2010 American Association for the Advancement of Science