Supplementary Materials

Supplementary Materials for:

PI3K Signaling Through the Dual GTPase–Activating Protein ARAP3 Is Essential for Developmental Angiogenesis

Laure Gambardella, Myriam Hemberger, Bethany Hughes, Enrique Zudaire, Simon Andrews, Sonja Vermeren*

*To whom correspondence should be addressed. E-mail: sonja.vermeren{at}bbsrc.ac.uk

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation of a conditional Arap3 knockout mouse.
  • Fig. S2. Loss of ARAP3 in the germ line, in embryonic tissues, or in the endothelial compartment, or incorporation of a PH domain point mutation causes defects in the embryo and yolk sac.
  • Fig. S3. Analysis of blood flow in Arap3–/– embryos.
  • Fig. S4. Analysis of the placental phenotype in Arap3 mutants.
  • Fig. S5. Arap3–/– embryos die due to an endothelial cell–autonomous vascular defect.

    Fig. S6. Vascular remodeling, but not hematopoiesis, is affected in Arap3–/–yolk sacs.
  • Fig. S7. Arap3+/– embryos show a mild, transient delay in angiogenesis.
  • Fig. S8. The Arap3–/– angiogenesis defect is not due to enhanced endothelial cell proliferation.
  • Fig. S9. Appearance of the allantois before explant culture.
  • Fig. S10. Analysis of sprouting angiogenesis.
  • Fig. S11. Generation of the Arap3R302,303A/R302,303A PH domain knock-in mouse.
  • Fig. S12. A PH domain point mutation in ARAP3, which uncouples it from PI3K, causes defective angiogenesis.
  • Fig. S13. ARAP3 knockdown in HUVECs.
  • Table S1. Genotypes of litters from Arap3+/– intercrosses.
  • Table S2. Genotypes of litters from Arap3+/R302,303A intercrosses.
  • References

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Citation: L. Gambardella, M. Hemberger, B. Hughes, E. Zudaire, S. Andrews, S. Vermeren, PI3K Signaling Through the Dual GTPase–Activating Protein ARAP3 Is Essential for Developmental Angiogenesis. Sci. Signal. 3, ra76 (2010).

© 2010 American Association for the Advancement of Science