Supplementary Materials

Supplementary Materials for:

Akt and Autophagy Cooperate to Promote Survival of Drug-Resistant Glioma

Qi-Wen Fan, Christine Cheng, Chris Hackett, Morri Feldman, Benjamin T. Houseman, Theodore Nicolaides, Daphne Haas-Kogan, C. David James, Scott A. Oakes, Jayanta Debnath, Kevan M. Shokat, William A. Weiss*

*To whom correspondence should be addressed. E-mail: weiss{at}cgl.ucsf.edu

This PDF file includes:

  • Fig. S1. Blockade of autophagosome maturation synergizes with inhibition of PI3K and mTOR to induce apoptosis in PTENmt glioma.
  • Fig. S2. PI-103 induces apoptosis in Atg5 mutant mouse embryo fibroblasts.
  • Fig. S3. Apoptosis in response to inhibition of mTOR and autophagy requires concomitant blockade of signaling through PI3K/Akt.
  • Fig. S4. Blockade of LAMP2 and Vps34 synergizes with inhibition of PI3K and mTOR to induce apoptosis in U373 PTENmt glioma.
  • Fig. S5. The ATP-competitive mTOR inhibitor Ku-0063794 induces autophagy more potently than does the allosteric mTORC1 inhibitor rapamycin and the PI3Kα inhibitor PIK-90 in PTENmt glioma.

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Citation: Q.-W. Fan, C. Cheng, C. Hackett, M. Feldman, B. T. Houseman, T. Nicolaides, D. Haas-Kogan, C. D. James, S. A. Oakes, J. Debnath, K. M. Shokat, W. A. Weiss, Akt and Autophagy Cooperate to Promote Survival of Drug-Resistant Glioma. Sci. Signal. 3, ra81 (2010).

© 2010 American Association for the Advancement of Science