Supplementary Materials

Supplementary Materials for:

The Mammalian MAPK/ERK Pathway Exhibits Properties of a

Negative Feedback Amplifier

Oliver E. Sturm, Richard Orton, Joan Grindlay, Marc Birtwistle, Vladislav Vyshemirsky, David Gilbert, Muffy Calder, Andrew Pitt, Boris Kholodenko, Walter Kolch*

*To whom correspondence should be addressed. E-mail: walter.kolch{at}

This PDF file includes:

  • Section 1. Models and modeling.
  • Section 2. Model parameters.
  • Section 3. SBML files of the models.
  • References
  • Fig. S1. Absolute concentrations of Raf-1, MEK, and ERK in COS1 and NIH 3T3 cells.
  • Fig. S2. Schematic topologies of the models used.
  • Fig. S3. Schematic of Raf-1 and the Raf-1 mutants used to probe the NFA hypothesis.
  • Fig. S4. Expression of Flag-tagged Raf-1 and the Raf6A mutant.
  • Fig. S5. The effects of U0126 on steady-state ppERK abundance with protein concentrations of COS1 cells.
  • Fig. S6. The NFA effect also stabilizes non-steady-state dynamic systems.
  • Fig. S7. Dose-dependent ERK activation by different stimuli.
  • Fig. S8. B-Raf kinase activity is not feedback-inhibited by ERK.

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Other Supplementary Material for this manuscript includes the following:


Citation: O. E. Sturm, R. Orton, J. Grindlay, M. Birtwistle, V. Vyshemirsky, D. Gilbert, M. Calder, A. Pitt, B. Kholodenko, W. Kolch, The Mammalian MAPK/ERK Pathway Exhibits Properties of a Negative Feedback Amplifier. Sci. Signal. 3, ra90 (2010).

© 2010 American Association for the Advancement of Science