Supplementary Materials

Supplementary Materials for:

Cyclic GMP and Protein Kinase G Control a Src-Containing
Mechanosome in Osteoblasts

Hema Rangaswami, Raphaela Schwappacher, Nisha Marathe, Shunhui Zhuang, Darren E. Casteel, Bodo Haas, Yong Chen, Alexander Pfeifer, Hisashi Kato, Sanford Shattil, Gerry R. Boss, Renate B. Pilz*

*To whom correspondence should be addressed. E-mail: rpilz{at}

This PDF file includes:

  • Materials and Methods
  • Fig. S1. siRNA knockdown of PKGI or PKGII; effects of fluid shear stress and cGMP on Src and ERK phosphorylation in MLO-Y4 and MC3T3 cells.
  • Fig. S2. PKGII does not directly phosphorylate Src.
  • Fig. S3. Effect of vanadate- and phosphatase-specific siRNAs on Src phosphorylation and dephosphorylation.
  • Fig. S4. Analysis of SHP-1 phosphorylation and function in MC3T3 cells.
  • Fig. S5. cGMP activation of Src requires ligation of β3 integrins.
  • Fig. S6. Colocalization of β3 integrins, PKGII, and Src with SHP-2–containing membrane complexes.
  • Fig. S7. Interactions among PKGII, SHP-2, Src, and β3 integrins.
  • Table S1. siRNA target sequences.
  • References

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Citation: H. Rangaswami, R. Schwappacher, N. Marathe, S. Zhuang, D. E. Casteel, B. Haas, Y. Chen, A. Pfeifer, H. Kato, S. Shattil, G. R. Boss, R. B. Pilz, Cyclic GMP and Protein Kinase G Control a Src-Containing Mechanosome in Osteoblasts. Sci. Signal. 3, ra91 (2010).

© 2010 American Association for the Advancement of Science