Supplementary Materials

Supplementary Materials for:

A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint

Shu Kondo and Norbert Perrimon*

*To whom correspondence should be addressed. E-mail: perrimon{at}receptor.med.harvard.edu

This PDF file includes:

  • Fig. S1. Caffeine-sensitive G2-M checkpoint of Drosophila S2R+ cells.
  • Fig. S2. Cell cycle profiles of cells depleted of the candidate genes.
  • Fig. S3. Secondary screen for mitotic exit defects.
  • Fig. S4. G2-M checkpoint defect of cells depleted of the candidate genes.
  • Fig. S5. DNA damage–induced phosphorylation of histone H2Av in cells depleted of candidate genes.
  • Fig. S6. Mitotic index and cell cycle profile of cells depleted of pre-IC proteins.
  • Table S1. Genes required for the G2-M checkpoint induced by doxorubicin.
  • Table S2. Primary hits excluded from further analysis and reasons for exclusion.
  • Table S3. Statistics of the secondary screens.
  • Table S4. Sequences of primers used for synthesizing dsRNA templates.
  • References

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Citation: S. Kondo, N. Perrimon, A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. Sci. Signal. 4, rs1 (2011).

© 2011 American Association for the Advancement of Science