Supplementary Materials

Supplementary Materials for:

Genome-Wide RNAi Screen Reveals Disease-Associated Genes That Are Common to Hedgehog and Wnt Signaling

Leni S. Jacob, Xiaofeng Wu, Michael E. Dodge, Chih-Wei Fan, Ozlem Kulak, Baozhi Chen, Wei Tang, Baolin Wang, James F. Amatruda, Lawrence Lum*

*To whom correspondence should be addressed. E-mail: lawrence.lum{at}UTSouthwestern.edu

This PDF file includes:

  • Fig. S1. Additional material relating to the RNAi screen.
  • Fig. S2. Confirmatory siRNA assays for gene function in Hh pathway response.
  • Fig. S3. Additional characterization of STK11–/– cells.
  • Fig. S4. Hh-induced Smo or Gli protein accumulation in the primary cilium is not affected in STK11–/– cells.
  • Fig. S5. Dvl phosphorylation is sensitive to IWP but not SAHA.
  • Fig. S6. Dvl2 undergoes a change in distribution in response to Wnt protein stimulation in MEFs.
  • Fig. S7. The defect in posterior morphogenesis and somite development is apparent in STK11 morphants stained for MyoD expression, which labels somitic tissue.
  • Fig. S8. Loss of STK11 in cancerous cells induces Dvl protein phosphorylation and compromises primary cilia formation.
  • Fig. S9. Cell line–dependent responses to Wnt ligand in cervical carcinoma cells lacking Stk11.
  • Fig. S10. Axin1 forms punctate structures in HeLa cells.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Dharmacon library primary screen results.
  • Table S2 (Microsoft Excel format). Qiagen library primary screening results.
  • Table S3 (Microsoft Excel format). Genes identified by two siRNA pools from the primary screen.
  • Table S4 (Microsoft Excel format). Counterscreen results.
  • Table S5 (Microsoft Excel format). siRNA sequences targeting disease genes.

Citation: L. S. Jacob, X. Wu, M.E. Dodge, C.-W. Fan, O. Kulak, B. Chen, W. Tang, B. Wang, J. F. Amatruda, L. Lum, Genome-Wide RNAi Screen Reveals Disease-Associated Genes That Are Common to Hedgehog and Wnt Signaling. Sci. Signal. 4, ra4 (2011).

© 2011 American Association for the Advancement of Science