Supplementary Materials

Supplementary Materials for:

Ubiquitination of K-Ras Enhances Activation and Facilitates Binding to Select Downstream Effectors

Atsuo T. Sasaki, Arkaitz Carracedo, Jason W. Locasale, Dimitrios Anastasiou, Koh Takeuchi, Emily Rose Kahoud, Sasson Haviv, John M. Asara, Pier Paolo Pandolfi, Lewis C. Cantley*

*To whom correspondence should be addressed. E-mail: lcantley{at}hms.harvard.edu

This PDF file includes:

  • Model of Ras signaling
  • Fig. S1. Characterization of ubiquitinated Ras.
  • Fig. S2. More Raf and PI3K bind to ubiquitinated Ras than to non-ubiquitinated Ras.
  • Fig. S3. Subcellular localization of ubiquitinated K-Ras.
  • Fig. S4. Anti-HA antibody, clone 16B12, faithfully recognizes HA-ubiquitin-Ras.
  • References

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Citation: A. T. Sasaki, A. Carracedo, J. W. Locasale, D. Anastasiou, K. Takeuchi, E. R. Kahoud, S. Haviv, J. M. Asara, P. P. Pandolfi, L. C. Cantley, Ubiquitination of K-Ras Enhances Activation and Facilitates Binding to Select Downstream Effectors. Sci. Signal. 4, ra13 (2011).

© 2011 American Association for the Advancement of Science