Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity
Fu-Sen Liang, Wen Qi Ho, Gerald R. Crabtree*
*To whom correspondence should be addressed. E-mail:
This PDF file includes:
- Fig. S1. Crystal structure of ABI1-(+)-ABA-PYL1 complex.
- Fig. S2. Dose response of ABA-induced luciferase activation in cultured mammalian cell lines.
- Fig. S3. Time course of luciferase activation by ABA or Rap in CHO cells.
- Fig. S4. Reversibility of ABA- and Rap-induced luciferase production in E14 cells under different washing conditions.
- Fig. S5. The amounts of fusion protein production in ABA and Rap system.
- Fig. S6. Luciferase induction by ABA in different cell types.
- Fig. S7. Distribution of GFP-PYLcs with or without ABA in HEK 293T cells.
- Fig. S8. Quantification of cytoplasmic localization of GFP-PYLcs.
- Fig. S9. ABA-induced nuclear localization of GFP-PYLcs.
- Fig. S10. ABA-induced membrane localization of GFP-PYLcs.
- Fig. S11. ABA-induced nuclear localization of Numb-GFP-PYLcs.
- Fig. S12. Orthogonal induction of luciferase by ABA and Rap in NIH 3T3 cells.
- Fig. S13. Independent ABA- or Rap-induced membrane localization of GFP fusion proteins.
- Fig. S14. Independent relocalization of GFP and mCherry fusion proteins by ABA and Rap systems.
- Fig. S15. Dose dependence of phosphatase activity of wild-type and mutant GST-ABIcs.
- Fig. S16. Quantification of GFP-PYLcs pull-down by wild-type or mutant ABI.
- Fig. S17. Luciferase activation by ABA-activator cassette with wild-type or mutant ABIcs in NIH 3T3 cells.
- Fig. S18. ABA stability in FBS.
- Fig. S19. Bioavailability of ABA in mice.
- Fig. S20. Effect of ABA on lymphocyte proliferation.
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Citation: F.-S. Liang, W. Q. Ho, G. R. Crabtree, Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity. Sci. Signal. 4, rs2 (2011).