Supplementary Materials

Sci. Signal., 10 May 2011
DOI: 10.1126/scisignal.2001258
Supplementary Materials for:

Methylation of a Phosphatase Specifies Dephosphorylation and Degradation of Activated Brassinosteroid Receptors

Guang Wu,* Xiuling Wang, Xianbin Li, Yuji Kamiya, Marisa S. Otegui, Joanne Chory*

*To whom correspondence should be addressed. E-mail: gwu3{at}wisc.edu (G.W.); chory{at}salk.edu (J.C.)

This PDF file includes:

  • Fig. S1. The abundance of bri1 was reduced in bri1-5, but bri1-5 phenotypes can be rescued by expression of additional bri1-5.
  • Fig. S2. Scheme for screening for suppressor of bri1-5.
  • Fig. S3. sbi1-dependent BRI1 or bri1-5 protein accumulation requires BRs.
  • Fig. S4. BRI1 or bri1-5 proteins in sbi1 are degraded in the absence of BRs.
  • Fig. S5. Scheme for map-cloning of SBI1.
  • Fig. S6. LCMTs are conserved in eukaryotes.
  • Fig. S7. SBI1(At1g02100) is expressed at similar levels as BRI1(At4g39400) and CPD(At5g05690) in young tissues and expression is higher than these two genes in mature organs.
  • Fig. S8. PP2Acs are conserved in eukaryotes.
  • Fig. S9. Methylated PP2Acs were undetectable in sbi1 mutant background.
  • Fig. S10. Distribution of BRI1 or bri1 in subcellular fractions.
  • Fig. S11. SBI1 partially localizes to membranous compartments.
  • Fig. S12. rcn1 and sbi1 mutants have similar phenotypes.
  • Fig. S13. SBI1 was specifically increased by BRs.
  • Reference

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Citation: G. Wu, X. Wang, X. Li, Y. Kamiya, M. S. Otegui, J. Chory, Methylation of a Phosphatase Specifies Dephosphorylation and Degradation of Activated Brassinosteroid Receptors. Sci. Signal. 4, ra29 (2011).

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