Supplementary Materials

Supplementary Materials for:

Ca2+ Signaling Tools Acquired from Prostasomes Are Required for Progesterone-Induced Sperm Motility

Kwang-Hyun Park, Byung-Ju Kim, John Kang, Tae-Sik Nam, Jung Min Lim, Hyoung Tae Kim, Jong Kwan Park, Young Gon Kim, Soo-Wan Chae, Uh-Hyun Kim*

*To whom correspondence should be addressed. E-mail: uhkim{at}chonbuk.ac.kr

This PDF file includes:

  • Fig. S1. ADPR-cyclase activity in sperm and semen of human subjects.
  • Fig. S2. Transfer of ADPR-cyclase activity by liquefaction.
  • Fig. S3. Progesterone concentration dependency of Ca2+ signaling in PFS.
  • Fig. S4. Occurrence of progesterone-induced Ca2+ oscillations.
  • Fig. S5. Characteristics of progesterone-induced Ca2+ signaling in PFS.
  • Fig. S6. Expression and transfer of Ca2+ signaling molecules in sperm.
  • Fig. S7. Progesterone-induced acrosome reaction in NFS and PFS.
  • Fig. S8. Effect of CD38 knockout on mouse fertilization.
  • Fig. S9. Characterization of a novel ADPR-cyclase from prostasomes.
  • Fig. S10. IUI before and after fusion of sperm with prostasomes.
  • Fig. S11. Expression of nongenomic membrane progesterone receptor in PFS.
  • Fig. S12. Comparison of progesterone-induced [Ca2+]i rise in noncapacitated and capacitated sperm.
  • Fig. S13. Effect of acidic condition on mouse epidydymal sperm motility.

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Citation: K.-H. Park, B.-J. Kim, J. Kang, T.-S. Nam, J. M. Lim, H. T. Kim, J. K. Park, Y. G. Kim, S.-W. Chae, U.-H. Kim, Ca2+ Signaling Tools Acquired from Prostasomes Are Required for Progesterone-Induced Sperm Motility. Sci. Signal. 4, ra31 (2011).

© 2011 American Association for the Advancement of Science