Supplementary Materials

Supplementary Materials for:

Sequential Phosphorylation of Smoothened Transduces Graded Hedgehog Signaling

Ying Su, Jason K. Ospina, Junzheng Zhang, Andrew P. Michelson, Adam M. Schoen, Alan Jian Zhu*

*To whom correspondence should be addressed. E-mail: zhua{at}

This PDF file includes:

  • Fig. S1. Hh signaling does not affect PKA or CKI abundance or activity.
  • Fig. S2. Phosphatase activity regulates Smo signaling in cl-8 cells.
  • Fig. S3. Phosphatase activity regulates the phosphorylation and surface localization of Smo in S2 cells.
  • Fig. S4. Alteration of Hh signaling activity by manipulating the activities of Smo-specific kinases or phosphatases during Drosophila wing development.
  • Fig. S5. Expression domains of dpp mRNA and a dpp-lacZ enhancer trap in a wild-type wing disc.
  • Fig. S6. Functional interaction between Ci and Tws-PP2A.
  • Fig. S7. Characterization of the phosphospecific antibody a-Smo-pS667.
  • Fig. S8. PP2A specifically dephosphorylates CKI-phosphorylated Smo.
  • Fig. S9. The role of PP4 in regulating Hh signaling.
  • Fig. S10. Distinct abilities of PP1 and PP2A to dephosphorylate moderately phosphorylated Smo.
  • Table S1. T7-primed primers used to construct the PSTP dsRNA library.

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Citation: Y. Su, J. K. Ospina, J. Zhang, A. P. Michelson, A. M. Schoen, A. J. Zhu, Sequential Phosphorylation of Smoothened Transduces Graded Hedgehog Signaling. Sci. Signal. 4, ra43 (2011).

© 2011 American Association for the Advancement of Science