Supplementary Materials

Supplementary Materials for:

A Cell-Based High-Content Screening Assay Reveals Activators and

Inhibitors of Cancer Cell Invasion

Manuela Quintavalle, Leonardo Elia, Jeffrey H. Price, Susanne Heynen-Genel, Sara A. Courtneidge*

*To whom correspondence should be addressed. E-mail: courtneidge{at}sanfordburnham.org

This PDF file includes:

  • Fig. S1. Statistics of positive (inhibited) and negative (uninhibited) control cell populations for rosette detection.
  • Fig. S2. Detailed analysis of the HCS results.
  • Fig. S3. Effect of purvalanol A on the cell cycle.
  • Fig. S4. p35 abundance and localization in Src-3T3 cells.
  • Fig. S5. Effect of Cdk5 knockdown on formation and function of invadopodia.
  • Fig. S6. Effect of purvalanol A on Cdk5 activity.
  • Fig. S7. Phosphorylation of caldesmon by Cdk5 regulates formation of invadopodia.
  • Fig. S8. Knockdown of caldesmon rescues formation of invadopodia in cells deficient in Cdk5.
  • Table S1. Statistics for the metrics used to determine cutoff values (wells flagged "cytotoxic/misdispensed").
  • Table S2. Statistics for the "Number of Detected Rosettes per Cell" metric used to determine active compound criteria.
  • Table S3. EC50 measurements for validated compounds.

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Citation: M. Quintavalle, L. Elia, J. H. Price, S. Heynen-Genel, S. A. Courtneidge, A Cell-Based High-Content Screening Assay Reveals Activators and Inhibitors of Cancer Cell Invasion. Sci. Signal. 4, ra49 (2011).

© 2011 American Association for the Advancement of Science