Supplementary Materials

Supplementary Materials for:

Distinct Phosphorylation Sites on the β2-Adrenergic Receptor Establish a Barcode That Encodes Differential Functions of β-Arrestin

Kelly N. Nobles, Kunhong Xiao,* Seungkirl Ahn, Arun K. Shukla, Christopher M. Lam, Sudarshan Rajagopal, Ryan T. Strachan, Teng-Yi Huang, Erin A. Bressler, Makoto R. Hara, Sudha K. Shenoy, Steven P. Gygi, Robert J. Lefkowitz*

*To whom correspondence should be addressed. E-mail: lefko001{at}receptor-biol.duke.edu (R.J.L.); khxiao{at}receptor-biol.duke.edu (K.X.)

This PDF file includes:

  • Fig. S1. Endogenous GRK2 and 6 in HEK293 cells.
  • Fig. S2. Specific β-arrestin2 recruitment to the β2AR.
  • Fig. S3. Overview of β2AR phosphorylation analysis by MS-based quantitative proteomics.
  • Fig. S4. Dephosphorylation of β2AR Ser246 by isoproterenol stimulation.
  • Fig. S5. Phosphorylation of Ser355/Ser356 on β2AR as detected by LC-MS/MS and Western blotting.
  • Fig. S6. Western blot analysis of phosphorylation of β2AR on Ser355/Ser356 in response to isoproterenol and carvedilol stimulation.
  • Fig. S7. Western blot analysis of β2AR phosphorylation at Ser396, Ser407/Ser411, Ser261/Ser262, and Ser345/Ser346.

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Citation: K. N. Nobles, K. Xiao, S. Ahn, A. K. Shukla, C. M. Lam, S. Rajagopal, R. T. Strachan, T.-Y. Huang, E. A. Bressler, M. R. Hara, S. K. Shenoy, S. P. Gygi, R. J. Lefkowitz, Distinct Phosphorylation Sites on the β2-Adrenergic Receptor Establish a Barcode That Encodes Differential Functions of β-Arrestin. Sci. Signal. 4, ra51 (2011).

© 2011 American Association for the Advancement of Science