Supplementary Materials

Supplementary Materials for:

Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal–Regulated Kinase Signaling

Adam A. Friedman, George Tucker, Rohit Singh, Dong Yan, Arunachalam Vinayagam, Yanhui Hu, Richard Binari, Pengyu Hong, Xiaoyun Sun, Maura Porto, Svetlana Pacifico, Thilakam Murali, Russell L. Finley Jr., John M. Asara, Bonnie Berger, Norbert Perrimon*

*To whom correspondence should be addressed. E-mail: perrimon{at}

This PDF file includes:

  • Fig. S1. Primary RNAi screen optimization and data.
  • Fig. S2. Performance of SAINT-based PPI ranking.
  • Fig. S3. Validation of additional PPI interactions.
  • Fig. S4. Phenotypes of additional RTK-Ras-ERK network components based on in vivo RNAi.
  • Table S3. RTK-specific hit subsets.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 50.2 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1. Compilation of all hits from six genome-wide RNAi screens performed in duplicate, and grouping by screen type.
  • Table S2. Gene Ontology Biological Process and Molecular Function categories enriched in RNAi screen hits and PPI data.
  • Table S4. Protein-protein interactions determined by TAP-MS analysis of RTK-Ras-ERK canonical pathway.
  • Table S5. Compilation of all in vivo results.
  • Table S6. Hairpin lines used for in vivo analyses.
  • Cytoscape file for the PPI and RNAi data.
  • Excel spreadsheets of peptides isolated in each TAP-MS experiment.
[Download Tables S1, S2, and S4 to S6 (Compressed)]

Technical Details

Format: Microsoft Excel

Size: 2.5 MB (compressed); 8.11 MB (decompressed)

[Download Cytoscape file (Compressed)]

Technical Details

Format: Cytoscape

Size: 41 KB (compressed); 42.3 MB (decompressed)

[Download Excel spreadsheets (Compressed)]

Technical Details

Format: Microsoft Excel

Size: 4.5 MB (compressed); 13.2 MB (decompressed)

Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; or StuffIt Expander (Windows and Mac;

Citation: A. A. Friedman, G. Tucker, R. Singh, D. Yan, A. Vinayagam, Y. Hu, R. Binari, P. Hong, X. Sun, M. Porto, S. Pacifico, T. Murali, R. L. Finley Jr., J. M. Asara, B. Berger, N. Perrimon, Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal–Regulated Kinase Signaling. Sci. Signal. 4, rs5 (2011).

© 2011 American Association for the Advancement of Science