Signaling by the Matrix Proteoglycan Decorin Controls Inflammation and Cancer Through PDCD4 and MicroRNA-21
Rosetta Merline, Kristin Moreth, Janet Beckmann, Madalina V. Nastase, Jinyang Zeng-Brouwers, José Guilherme Tralhão, Patricia Lemarchand, Josef Pfeilschifter, Roland M. Schaefer, Renato V. Iozzo, Liliana Schaefer*
*To whom correspondence should be addressed. E-mail:
This PDF file includes:
- Fig. S1. LPS-mediated increase in decorin abundance in macrophages in vivo and in vitro.
- Fig. S2. Role of negative feedback signaling through the IL-10 receptor in the effects of decorin and LPS on TNFα and IL-12p70 protein and mRNA abundance in vivo and in vitro.
- Fig. S3. Decorin treatment triggers the phosphorylation of the MAPKs p44p42 and p38 in thioglycolate-elicited macrophages in a TLR2- and TLR4-dependent manner.
- Fig. S4. Analysis of decorin binding to recombinant TLR2 and to the TLR4-MD2 complex with microscale thermophoresis.
- Fig. S5. Procedures to rule out contamination of decorin.
- Fig. S6. Decorin- and LPS-dependent regulation of IL-10 release is mediated by miR-21 and PDCD4 in thioglycolate-elicited macrophages.
- Fig. S7. Decorin-dependent regulation of Pdcd4 expression in LPS-induced septic spleen and lungs and in Ad.DCN-transfected tumor xenografts.
- Table S1. Characteristics of patients with sepsis and healthy controls.
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Citation: R. Merline, K. Moreth, J. Beckmann, M. V. Nastase, J. Zeng-Brouwers, J. G. Tralhão, P. Lemarchand, J. Pfeilschifter, R. M. Schaefer, R. V. Iozzo, L. Schaefer, Signaling by the Matrix Proteoglycan Decorin Controls Inflammation and Cancer Through PDCD4 and MicroRNA-21. Sci. Signal. 4, ra75 (2011).