Supplementary Materials

Supplementary Materials for:

Ric-8 Proteins Are Molecular Chaperones That Direct Nascent G Protein α Subunit Membrane Association

Meital Gabay, Mary E. Pinter, Forrest A. Wright, PuiYee Chan, Andrew J. Murphy, David M. Valenzuela, George D. Yancopoulos, Gregory G. Tall*

*To whom correspondence should be addressed. E-mail: gregory_tall{at}urmc.rochester.edu

This PDF file includes:

  • Fig. S1. Genomic PCR scheme to genotype Ric-8A– and Ric-8B–deleted mice and derived mES cell lines.
  • Fig. S2. Increased abundance of Gβγ does not rescue the defects in Gα protein abundance observed in Ric-8–/–cells.
  • Fig. S3. Nascent Gαi appears to be differentially modified in Ric-8A–/– and wild-type cells by means other than myristoylation.
  • Fig. S4. Forskolin-stimulated cAMP production is blunted in Ric-8A–/– and Ric-8B–/– cells compared to that in wild-type cells.

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Citation: M. Gabay, M. E. Pinter, F. A. Wright, P. Chan, A. J. Murphy, D. M. Valenzuela, G. D. Yancopoulos, G. G. Tall, Ric-8 Proteins Are Molecular Chaperones That Direct Nascent G Protein α Subunit Membrane Association. Sci. Signal. 4, ra79 (2011).

© 2011 American Association for the Advancement of Science