Supplementary Materials

Supplementary Materials for:

i2 Signaling Promotes Skeletal Muscle Hypertrophy, Myoblast Differentiation, and Muscle Regeneration

Giulia C. Minetti, Jerome N. Feige, Antonia Rosenstiel, Florian Bombard, Viktor Meier, Annick Werner, Frederic Bassilana, Andreas W. Sailer, Peter Kahle, Christian Lambert, David J. Glass,* Mara Fornaro*

*To whom correspondence should be addressed. E-mail: mara.fornaro{at}novartis.com (M.F.); david.glass{at}novartis.com (D.J.G.)

This PDF file includes:

  • Fig. S1. LPA and constitutively active Gαi2 inhibit the cAMP-CREB pathway.
  • Fig. S2. Abundance of Gαi2, PGC-1α, and PPARβ/σ in tibialis muscle infected with AAV-Gαi2 (Q205L) or AAV-EV.
  • Fig. S3. Rapamycin but not LY294002 blocks hypertrophy induced by expression of constitutively active Gai2 in C2C12 myotubes.
  • Fig. S4. LPA and Gαi2 (Q205L) induce skeletal myotube hypertrophy of HuSkMC myotubes through a PKC-mediated pathway.
  • Fig. S5. Gαi2 promotes satellite cell activation and differentiation.
  • Fig. S6. Expression of constitutively active Gαi2 promotes nuclear export of HDAC4.

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Citation: G. C. Minetti, J. N. Feige, A. Rosenstiel, F. Bombard, V. Meier, A. Werner, F. Bassilana, A. W. Sailer, P. Kahle, C. Lambert, D. J. Glass, M. Fornaro, A-D. Maturation, Gαi2 Signaling Promotes Skeletal Muscle Hypertrophy, Myoblast Differentiation, and Muscle Regeneration. Sci. Signal. 4, ra80 (2011).

© 2011 American Association for the Advancement of Science