Supplementary Materials

Supplementary Materials for:

RGS Proteins Maintain Robustness of GPCR-GIRK Coupling by Selective Stimulation of the G Protein Subunit Gαo

Huai-hu Chuang* and Alexander Y. Chuang

*To whom correspondence should be addressed. E-mail: huai-hu.chuang{at}cornell.edu

This PDF file includes:

  • Materials and Methods
  • Mathematical simulation of G protein activation for Fig. 1A
  • Membrane-targeted RGS constructs
  • Fig. S1. Deactivation kinetics of A1 or m2 for various RGS proteins.
  • Fig. S2. RGS4 does not stimulate β2-AR–stimulated Gαs-dependent GIRK currents.
  • Fig. S3. Deactivation kinetics of membrane-associated RGS box constructs.
  • Fig. S4. Deactivation kinetics and ACh dose-response curves of oocytes with or without the full-length RGS4N128A.
  • Fig. S5. Deactivation kinetics of the lyn-tagged R4Box and mutants.
  • Fig. S6. Effects of PTX treatment.
  • Fig. S7. RGS expression level–dependent deactivation kinetics.
  • Fig. S8. Increased RGS8 expression enhanced the acceleration of deactivation kinetics.
  • Fig. S9. GTP-γ-S induced GIRK activation in Gαi1-expressing oocyte membranes.
  • Fig. S10. RGS4 exhibited its GAP effect on Gαi1 R178M.
  • Table S1. P values for comparison of RGS effects on current enhancement in Fig. 1D.
  • Table S2. P values for stimulatory effects on GIRK current by various membrane-targeted RGS box constructs.
  • References

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Citation: H.-h. Chuang, A. Y. Chuang, RGS Proteins Maintain Robustness of GPCR-GIRK Coupling by Selective Stimulation of the G Protein Subunit Gαo. Sci. Signal. 5, ra15 (2012).

© 2012 American Association for the Advancement of Science