Supplementary Materials

Supplementary Materials for:

Diverse Sensitivity Thresholds in Dynamic Signaling Responses by Social Amoebae

C. Joanne Wang, Adriel Bergmann, Benjamin Lin, Kyuri Kim, Andre Levchenko*

*To whom correspondence should be addressed. E-mail: alev{at}

This PDF file includes:

  • Text S1. Determining the threshold for partitioning responders from nonresponders.
  • Text S2. Comparing the responses of single integral feedback and feedforward loops to ramp input.
  • Text S3. Method for gradient sensing analysis.
  • Text S4. Setup and development of the collective cell response model.
  • Text S5. Mathematical description of the aLEGI model.
  • Fig. S1. The microfluidic function generator.
  • Fig. S2. The properties of aLEGI model.
  • Fig. S3. Schematic mechanism of increased rate of cAMP wave propagation as a function of the colony heterogeneity.
  • Fig. S4. Simulation of the average dose-response dynamics.
  • Fig. S5. Dependence of PIP3 responses on probe expression.
  • Fig. S6. Distribution of cell responses to step stimulation at two different developmental stages.
  • Fig. S7. Responses after latrunculin A treatment.
  • References
  • Movies S1 to S14 legends

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Other Supplementary Material for this manuscript includes the following:

  • Movies S1 to S14 (.avi format). Translocation kinetics of PHcrac-GFP in cells stimulated under various conditions.

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Citation: C. J. Wang, A. Bergmann, B. Lin, K. Kim, A. Levchenko, Diverse Sensitivity Thresholds in Dynamic Signaling Responses by Social Amoebae. Sci. Signal. 5, ra17 (2012).

© 2012 American Association for the Advancement of Science