Supplementary Materials

Supplementary Materials for:

Astrocytes Modulate Neural Network Activity by Ca2+-Dependent

Uptake of Extracellular K+

Fushun Wang, Nathan A. Smith, Qiwu Xu, Takumi Fujita, Akemichi Baba, Toshio Matsuda, Takahiro Takano, Lane Bekar, Maiken Nedergaard*

*To whom correspondence should be addressed. E-mail: nedergaard{at}urmc.rochester.edu

This PDF file includes:

  • Fig. S1. Ouabain-insensitive 86Rb+ uptake.
  • Fig. S2. Doxycycline suppresses eGFP expression in cultured astrocytes prepared from MrgA1+/− mice.
  • Fig. S3. GPCR-activated increases in 86Rb+ uptake require increases in cytosolic Ca2+ in cultured astrocytes.
  • Fig. S4. Comparison of 86Rb+ uptake in rat versus mouse and rat cortical versus hippocampal astrocytes.
  • Fig. S5. Detection of GPCR-activated changes in EPSPs is depth- and [K+]Bath-dependent.
  • Fig. S6. GPCR activation triggers a transient minor increase in intra-astrocytic Na+ concentration and a minor decrease in extracellular Ca2+ concentration in hippocampal slices.
  • Fig. S7. Activation of GPCR-induced changes in PKA and PKC activity does not mediate ATP-induced increases in 86Rb+ uptake in cultured astrocytes.
  • Fig. S8. Combined recordings of extracellular K+ with mEPSCs or mIPSCs in hippocampal slices.
  • Fig. S9. Effects of GPCR activation or reduction of bath K+ concentration on extracellular K+, neuronal membrane potential, and evoked EPSP in hippocampal slices.

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Citation: F. Wang, N. A. Smith, Q. Xu, T. Fujita, A. Baba, T. Matsuda, T. Takano, L. Bekar, M. Nedergaard, Astrocytes Modulate Neural Network Activity by Ca2+-Dependent Uptake of Extracellular K+. Sci. Signal. 5, ra26 (2012).

© 2012 American Association for the Advancement of Science