Supplementary Materials

Supplementary Materials for:

A Large Bioactive BMP Ligand with Distinct Signaling Properties Is Produced by Alternative Proconvertase Processing

Takuya Akiyama, Guillermo Marqués, Kristi A. Wharton*

*To whom correspondence should be addressed. E-mail: kristi_wharton{at}brown.edu

This PDF file includes:

  • Fig. S1. Signaling activity of the HA-tagged Gbb constructs.
  • Fig. S2. Gbb38 and Gbb15 bind to the Sax type I receptor.
  • Fig. S3. Signaling activity of the hBMP4 and hBMP4 cleavage mutants.
  • Fig. S4. Failure to cleave Gbb at the NS results in wing defects.
  • Fig. S5. The gbb double cleavage mutant exhibits dominant-negative behavior when expressed in dpp-expressing cells of the wing imaginal disc.
  • Fig. S6. Wild-type gbb (Gbb38 and Gbb15) and gbbmS1 (Gbb38) exhibit nonautonomous signaling.
  • Table S1. Macrochaetae and leg defects associated with misexpression of gbb and gbb cleavage mutants in dpp-expressing cells.
  • Table S2. Primers used in this study.

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Citation: T. Akiyama, G. Marqués, K. A. Wharton, A Large Bioactive BMP Ligand with Distinct Signaling Properties Is Produced by Alternative Proconvertase Processing. Sci. Signal. 5, ra28 (2012).

© 2012 American Association for the Advancement of Science