Supplementary Materials

Supplementary Materials for:

Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca2+ and cAMP Signaling

Debbie Willoughby, Katy L. Everett, Michelle L. Halls, Jonathan Pacheco, Philipp Skroblin, Luis Vaca, Enno Klussmann, Dermot M. F. Cooper*

*To whom correspondence should be addressed. E-mail: dmfc2{at}cam.ac.uk

This PDF file includes:

  • Fig. S1. Constructs used to study AC8 and Orai1 interaction.
  • Fig. S2. Colocalization between Orai1 and cholera toxin B subunit is enhanced following thapsigargin treatment.
  • Fig. S3. Relative levels of GST-tagged AC8 fragments.
  • Fig. S4. Specificity of Orai1 and STIM1 antibodies and Orai1 knockdown.
  • Fig. S5. Spot sequences for peptide array data presented in Fig. 4.
  • Fig. S6. Isolation of the ionomycin-induced, nonspecific Ca2+ entry signal in GCaMP2-AC8– and global GCaMP2–expressing cells.

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Citation: D. Willoughby, K. L. Everett, M. L. Halls, J. Pacheco, P. Skroblin, L. Vaca, E. Klussmann, D. M. F. Cooper, Direct Binding Between Orai1 and AC8 Mediates Dynamic Interplay Between Ca2+ and cAMP Signaling. Sci. Signal. 5, ra29 (2012).

© 2012 American Association for the Advancement of Science