Supplementary Materials

Supplementary Materials for:

Synthetic Signal Propagation Through Direct Cell-Cell Interaction

Mitsuhiro Matsuda, Makito Koga, Eisuke Nishida, Miki Ebisuya*

*To whom correspondence should be addressed. E-mail: ebisuya{at}

This PDF file includes:

  • Fig. S1. Quantification of endogenous Delta and Notch transcripts.
  • Fig. S2. Characterization of the genetic circuits.
  • Fig. S3. Bistability and signal propagation.
  • Fig. S4. The ability of Delta-2A–GFP to function as a ligand for Notch.
  • Legends for Movies S1 and S2

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.18 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.MPG format). Time-lapse imaging of signal propagation.
  • Movie S2 (.MPG format). Time-lapse imaging of signal propagation using the cells aligned in a row.

[Download Movies S1 and S2 (Compressed)]

Technical Details

Format: MPG. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 2.42 MB (compressed); 2.46 MB (decompressed)

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; or StuffIt Expander (Windows and Mac;

Citation: M. Matsuda, M. Koga, E. Nishida, M. Ebisuya, Synthetic Signal Propagation Through Direct Cell-Cell Interaction. Sci. Signal. 5, ra31 (2012).

© 2012 American Association for the Advancement of Science