Supplementary Materials

Supplementary Materials for:

Differential β-Arrestin–Dependent Conformational Signaling and Cellular Responses Revealed by Angiotensin Analogs

Brandon Zimmerman, Alexandre Beautrait, Benjamin Aguila, Ricardo Charles, Emanuel Escher, Audrey Claing, Michel Bouvier,* Stéphane A. Laporte*

*To whom correspondence should be addressed. E-mail: michel.bouvier{at}umontreal.ca (M.B.); stephane.laporte{at}mcgill.ca (S.A.L.)

This PDF file includes:

  • Fig. S1. Analog-induced production of IP1 and recruitment of β-arrestin as analyzed by confocal microscopy and immunoprecipitation.
  • Fig. S2. Recruitment of β-arrestin to AT1R and conformational changes induced by AngII analogs.
  • Fig. S3. siRNA-mediated knockdown of GRK2 and GRK6.
  • Fig. S4. Role of GRK2 and GRK6 in the efficacy and potency on ligand-induced β- arrestin recruitment and conformational change.
  • Fig. S5. Dose-response relationship of ERK1/2 activation by AngII and analogs.
  • Fig. S6. Correlation plots between ligand affinity, BRET values, and ERK1/2 activation.
  • Table S1. IP1 dose-response curve data used to calculate σ values and β factors.
  • Table S2. BRET dose-response data used to calculate σ values and β factors.

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Citation: B. Zimmerman, A. Beautrait, B. Aguila, R. Charles, E. Escher, A. Claing, M. Bouvier, S. A. Laporte, Differential β-Arrestin–Dependent Conformational Signaling and Cellular Responses Revealed by Angiotensin Analogs. Sci. Signal. 5, ra33 (2012).

© 2012 American Association for the Advancement of Science