Supplementary Materials

Supplementary Materials for:

Studying the Dynamics of SLP-76, Nck, and Vav1 Multimolecular Complex Formation in Live Human Cells with Triple-Color FRET

Maor H. Pauker, Nirit Hassan, Elad Noy, Barak Reicher, Mira Barda-Saad*

*To whom correspondence should be addressed. E-mail: Mira.Barda-Saad{at}biu.ac.il

This PDF file includes:

  • Fig. S1. Western blotting analysis of single-labeled cell lines and of positive and negative control cells for 3FRET.
  • Fig. S2. Flow cytometric analysis of single-labeled cell lines and of positive and negative control cells for 3FRET.
  • Fig. S3. FRET analysis of the interactions between Vav1, Nck, and SLP-76.
  • Legend for Movies S1 and S2
  • Table S1. Channels used for FRET analysis.
  • Table S2. Cell lines used as FRET controls.
  • Table S3. 2FRET analysis of the interaction between Nck and Vav1 in the presence or absence of wild-type SLP-76.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Dynamics of Nck, Vav1, and wild-type SLP-76 in live T cells.
  • Movie S2 (.mov format). Dynamics of Nck, Vav1, and the SLP-76 Y3F mutant in live T cells.

[Download Movies S1 and S2 (Compressed)]

Technical Details

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Citation: M. H. Pauker, N. Hassan, E. Noy, B. Reicher, M. Barda-Saad, Studying the Dynamics of SLP-76, Nck, and Vav1 Multimolecular Complex Formation in Live Human Cells with Triple-Color FRET. Sci. Signal. 5, rs3 (2012).

© 2012 American Association for the Advancement of Science