Supplementary Materials

Supplementary Materials for:

Sequence-Specific Recognition of a PxLPxI/L Motif by an Ankyrin Repeat Tumbler Lock

Chao Xu, Jing Jin, Chuanbing Bian, Robert Lam, Ruijun Tian, Ryan Weist, Linya You, Jianyun Nie, Alexey Bochkarev, Wolfram Tempel, Chris S. Tan, Gregory A. Wasney, Masoud Vedadi, Gerald D. Gish, Cheryl H. Arrowsmith, Tony Pawson, Xiang-Jiao Yang, Jinrong Min*

*To whom correspondence should be addressed. E-mail: jr.min{at}utoronto.ca

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Sequence of the peptides on the spot array.
  • Fig. S2. Crystal structures of apo-ANKRA2 and its complexes with the megalin peptides.
  • Fig. S3. Sequence alignment and structural comparison of ANKRA2 and RFXANK.
  • Fig. S4. Arg199 of RFXANK is critical to determining its ligand-binding specificity.
  • Fig. S5. Detailed electrospray ionization (ESI)–MS/MS spectrum analysis of pSer350 of HDAC4.
  • Fig. S6. Resemblance among repeat-type domains with respect to their ligandbinding modes.
  • Table S1. Data collection and refinement statistics.
  • References

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Citation: C. Xu, J. Jin, C. Bian, R. Lam, R. Tian, R. Weist, L. You, J. Nie, A. Bochkarev, W. Tempel, C. S. Tan, G. A. Wasney, M. Vedadi, G. D. Gish, C. H. Arrowsmith, T. Pawson, X.-J. Yang, J. Min, Sequence-Specific Recognition of a PxLPxI/L Motif by an Ankyrin Repeat Tumbler Lock. Sci. Signal. 5, ra39 (2012).

© 2012 American Association for the Advancement of Science