Supplementary Materials

Supplementary Materials for:

Histone Deacetylases 6 and 9 and Sirtuin-1 Control Foxp3+ Regulatory T Cell Function Through Shared and Isoform-Specific Mechanisms

Ulf H. Beier, Liqing Wang, Rongxiang Han, Tatiana Akimova, Yujie Liu, Wayne W. Hancock*

*To whom correspondence should be addressed. E-mail: whancock{at}mail.med.upenn.edu

This PDF file includes:

  • Fig. S1. ACY-738 and Tubastatin are HDAC6 inhibitors.
  • Fig. S2. Immunofluorescence analysis of Tregs to assess the cytosolic and nuclear localization of Foxp3.
  • Fig. S3. Western blotting analysis of Treg lysates to compare Foxp3 transcription factors.
  • Fig. S4. Loss of Sirt1 does not affect conversion to iTregs.
  • Fig. S5. Loss of HDAC9 does not alter TSDR methylation.
  • Fig. S6. Purity control of the effector T cells used for the pyrosequencing methylation assay (fig. S5).
  • Fig. S7. Combined deletion of Sirt1 and HDAC9 produces minor improvements in Treg function.
  • Fig. S8. Targeting of three HDACs does not improve Treg function any more than does use of dual HDACi.
  • Fig. S9. Proliferation of effector T cells is not affected by HDAC inhibitors.
  • Fig. S10. Examples of image processing.
  • Table S1. Statistical analysis for Fig. 1C.
  • Table S2. Statistical analysis for Fig. 1G.

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Citation: U. H. Beier, L. Wang, R. Han, T. Akimova, Y. Liu, W. W. Hancock, Histone Deacetylases 6 and 9 and Sirtuin-1 Control Foxp3+ Regulatory T Cell Function Through Shared and Isoform-Specific Mechanisms. Sci. Signal. 5, ra45 (2012).

© 2012 American Association for the Advancement of Science