Supplementary Materials

Supplementary Materials for:

Differential RET Signaling Pathways Drive Development of the Enteric Lymphoid and Nervous Systems

Amisha Patel, Nicola Harker, Lara Moreira-Santos, Manuela Ferreira, Kieran Alden, Jon Timmis, Katie Foster, Anna Garefalaki, Panayotis Pachnis, Paul Andrews, Hideki Enomoto, Jeffrey Milbrandt, Vassilis Pachnis, Mark C. Coles, Dimitris Kioussis, Henrique Veiga-Fernandes*

*To whom correspondence should be addressed. E-mail: jhfernandes{at}fm.ul.pt

This PDF file includes:

  • Methods
  • Fig. S1. Stroma cell activation, LTi and LTin cell frequencies, chemokine blocking, lymphotoxin modulation, and A test results for chemokine simulations.
  • Fig. S2. RET co-receptors in the gut.
  • Fig. S3. GDNF and GFRα1 signaling in lymphoid organ formation.
  • Fig. S4. Neural projections induced by GFLs.
  • Fig. S5. PP development in GFRα1 cis mice.
  • Fig. S6. Activity diagram of cellular events leading to PP triggering.
  • Fig. S7. State diagram of LTin cells.
  • Fig. S8. State diagram of LTi cells.
  • Fig. S9. State diagram of LTo cells.
  • Fig. S10. State diagram of non-LTo RET ligand producer cells.
  • Fig. S11. Parameter descriptions.
  • Fig. S12. Activity diagram of trans RET signaling events leading to PP formation.
  • Fig. S13. State diagram of LTin cells when RET signaling in trans is used.
  • Fig. S14. State diagram of LTi cells when RET signaling in trans is used.
  • Fig. S15. State diagram of LTo cells when RET signaling in trans is used.
  • Fig. S16. State diagram of non-LTo RET ligand producer cells for in trans RET signaling.
  • Movies S1 to S4 captions

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Motility of hematopoietic GFP+ cells in explanted gut cultures with ARTN-soaked beads.
  • Movie S2 (.mov format). Motility of hematopoietic GFP+ cells in explanted gut cultures with BSA-soaked beads.
  • Movie S3 (.mov format). Motility of hematopoietic cells in silico using RET signaling in cis.
  • Movie S4 (.mov format). Motility of hematopoietic cells in silico using RET signaling in trans.
  • Simulation code

 


Citation: A. Patel, N. Harker, L. Moreira-Santos, M. Ferreira, K. Alden, J. Timmis, K. Foster, A. Garefalaki, P. Pachnis, P. Andrews, H. Enomoto, J. Milbrandt, V. Pachnis, M. C. Coles, D. Kioussis, H. Veiga-Fernandes, Differential RET Signaling Pathways Drive Development of the Enteric Lymphoid and Nervous Systems. Sci. Signal. 5, ra55 (2012).

© 2012 American Association for the Advancement of Science