The Protease Omi Cleaves the Mitogen-Activated Protein Kinase Kinase MEK1 to Inhibit Microglial Activation
Qingsong Hu, Bin Li, Ranjie Xu, Dong Chen, Chenchen Mu, Erkang Fei, Guanghui Wang*
*To whom correspondence should be addressed. E-mail:
This PDF file includes:
- Fig. S1. The amounts of pERK1/2 proteins in the brains of mnd2 mice are increased compared with those of age-matched wild-type mice.
- Fig. S2. Omi protein abundance is unaffected by transfection or control siRNA.
- Fig. S3. The protease activity of Omi is required for the decrease in EGFP-MEK1 abundance.
- Fig. S4. MEK1 directly interacts with Omi in vitro.
- Fig. S5. Omi cleaves the substrate β-casein in vitro.
- Fig. S6. PD98059 blocks the decrease in the abundance of IκBα that is induced by knockdown of Omi.
- Fig. S7. Analysis of cytokine mRNA abundances in the brains of mnd2 mice.
Format: Adobe Acrobat PDF
Size: 126 KB
Citation: Q. Hu, B. Li, R. Xu, D. Chen, C. Mu, E. Fei, G. Wang, The Protease Omi Cleaves the Mitogen-Activated Protein Kinase Kinase MEK1 to Inhibit Microglial Activation. Sci. Signal. 5, ra61 (2012).