Supplementary Materials

Supplementary Materials for:

The Complex of G Protein Regulator RGS9-2 and Gβ5 Controls Sensitization and Signaling Kinetics of Type 5 Adenylyl Cyclase in the Striatum

Keqiang Xie, Ikuo Masuho, Cameron Brand, Carmen W. Dessauer, Kirill A. Martemyanov*

*To whom correspondence should be addressed. E-mail: kirill{at}scripps.edu

This PDF file includes:

  • Fig. S1. The N364H mutation abolishes GTPase activity of RGS9-2 and its effects on G protein subunit reassociation.
  • Fig. S2. Absence of AC superactivation by chronic stimulation of µ-ORs in HEK293T cells lacking AC5.
  • Fig. S3. Equal expression of wild-type RGS9-2 and its N364H mutant upon expression in transfected HEK293 cells.
  • Fig. S4. Absence of a specific BRET signal in cells not transfected with µ-OR or Gα.
  • Fig. S5. Uniform expression of Gα subunits in the BRET assay system.
  • Fig. S6. Time course of changes in cAMP concentration induced by isoproterenol in transfected HEK293T cells.

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Citation: K. Xie, I. Masuho, C. Brand, C. W. Dessauer, K. A. Martemyanov, The Complex of G Protein Regulator RGS9-2 and Gβ5 Controls Sensitization and Signaling Kinetics of Type 5 Adenylyl Cyclase in the Striatum. Sci. Signal. 5, ra63 (2012).

© 2012 American Association for the Advancement of Science