Supplementary Materials

Supplementary Materials for:

Key Roles for the Lipid Signaling Enzyme Phospholipase D1 in the Tumor Microenvironment During Tumor Angiogenesis and Metastasis

Qin Chen, Tsunaki Hongu, Takanobu Sato, Yi Zhang, Wahida Ali, Julie-Ann Cavallo, Adrianus van der Velden, Huasong Tian, Gilbert Di Paolo, Bernhard Nieswandt, Yasunori Kanaho, Michael A. Frohman*

*To whom correspondence should be addressed. E-mail: michael{at}pharm.stonybrook.edu

This PDF file includes:

  • Fig. S1. Generation and characterization of PLD1-deficient mice.
  • Fig. S2. Tumor growth is comparable in Pld1+/–, Pld2–/–, and wild-type mice.
  • Fig. S3. Macrophage infiltration is comparable in wild-type and Pld1–/– tumors.
  • Fig. S4. Tumor angiogenesis is comparable in Pld2–/– and wild-type mice.
  • Fig. S5. Pld1–/– and wild-type lung endothelial cells proliferate at comparable rates.
  • Fig. S6. VEGFR2 abundance is comparable in wild-type and Pld1–/– endothelial cells.
  • Fig. S7. FIPI-treated mice do not exhibit weight loss.
  • Fig. S8. JON/A inhibits activation of αIIbβ3 integrin and tumor metastasis.

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Citation: Q. Chen, T. Hongu, T. Sato, Y. Zhang, W. Ali, J.-A. Cavallo, A. van der Velden, H. Tian, G. Di Paolo, B. Nieswandt, Y. Kanaho, M. A. Frohman, Key Roles for the Lipid Signaling Enzyme Phospholipase D1 in the Tumor Microenvironment During Tumor Angiogenesis and Metastasis. Sci. Signal. 5, ra79 (2012).

© 2012 American Association for the Advancement of Science