Supplementary Materials

Sci. Signal., 20 November 2012
DOI: 10.1126/scisignal.2003431
Supplementary Materials for:

SUMOylation Silences Heterodimeric TASK Potassium Channels Containing K2P1 Subunits in Cerebellar Granule Neurons

Leigh D. Plant, Leandro Zuniga, Dan Araki, Jeremy D. Marks, Steve A. N. Goldstein*

*To whom correspondence should be addressed. E-mail: goldstein{at}brandeis.edu

This PDF file includes:

  • Fig. S1. KCNK1, KCNK3, and KCNK9 transcripts are present together in single cerebellar granule neurons.
  • Fig. S2. Antibodies to K2P1, K2P3, and K3P9 are specific.
  • Fig. S3. Antibody-mediated FRET demonstrates that interaction between K2P9 and SUMO1 requires K2P1.
  • Fig. S4. FRET indicates that K2P2 does not interact with K2P1, K2P3, K2P9, or SUMO1.
  • Fig. S5. Whole-cell recording of K2P1 expressed with K2P3 or K2P9 in CHO cells.
  • Fig. S6. Silencing by SUMO1 and SUMO2 is independent of the order of K2P subunits in tandem constructs.
  • Fig. S7. Studies of K2P1-Y231F subunits expressed in CHO cells or cerebellar granule neurons.
  • Table S1. Biophysical parameters for cerebellar granule neurons.
  • Table S2. Donor decay time constants indicate those K2P subunits that interact.
  • Table S3. Donor decay time constants indicate that K2P1 is required for interaction of YFP-SUMO1 and CFP-K2P3 or CFP-K2P9 in CHO cells and cerebellar granule neurons.
  • Movie S1 legend

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Projection of fluorescently labeled KCNK transcripts in a single cerebellar granule neuron.

Citation: L. D. Plant, L. Zuniga, D. Araki, J. D. Marks, S. A. N. Goldstein, SUMOylation Silences Heterodimeric TASK Potassium Channels Containing K2P1 Subunits in Cerebellar Granule Neurons. Sci. Signal. 5, ra84 (2012).

© 2012 American Association for the Advancement of Science

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