Supplementary Materials

Supplementary Materials for:

Mechanism of Stretch-Induced Activation of the Mechanotransducer Zyxin in Vascular Cells

Sahana Suresh Babu, Agnieszka Wojtowicz, Marc Freichel, Lutz Birnbaumer, Markus Hecker,* Marco Cattaruzza

*To whom correspondence should be addressed. E-mail: hecker{at}physiologie.uni-hd.de

This PDF file includes:

  • Fig. S1. Stretch-induced, zyxin-dependent gene expression.
  • Fig. S2. Technical controls and standards.
  • Fig. S3. Expression of natriuretic peptides by human umbilical vein endothelial cells.
  • Fig. S4. Representative immunofluorescence analyses of samples used for quantitative representations.
  • Fig. S5. Western blotting analysis of stretch-induced zyxin translocation from focal adhesions (cytosolic fraction) to the nucleus (nuclear fraction) in cells after subcellular fractionation.
  • Fig. S6. Expression of TRP channels in endothelial cells.
  • Fig. S7. TRPC3 or TRPC6 mediates stretch-induced zyxin activation.
  • Fig. S8. Characterization of the effect of TRPC3 inhibition on stretch-induced responses.
  • Fig. S9. Phosphorylation of the stretch-sensitive residue Ser142 is necessary for ANP-induced nuclear translocation of zyxin.
  • Fig. S10. Wall tension–induced zyxin signaling in lung epithelial and bladder smooth muscle cells.
  • Table S1. Synthetic oligonucleotides used for cloning and for real-time RT-PCR analysis.

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Citation: S. S. Babu, A. Wojtowicz, M. Freichel, L. Birnbaumer, M. Hecker, M. Cattaruzza, Mechanism of Stretch-Induced Activation of the Mechanotransducer Zyxin in Vascular Cells. Sci. Signal. 5, ra91 (2012).

© 2012 American Association for the Advancement of Science