Supplementary Materials

Supplementary Materials for:

Cycles of Ubiquitination and Deubiquitination Critically Regulate Growth Factor–Mediated Activation of Akt Signaling

Wei-Lei Yang, Guoxiang Jin, Chien-Feng Li, Yun Seong Jeong, Asad Moten, Dazhi Xu, Zizhen Feng, Wei Chen, Zhen Cai, Bryant Darnay, Wei Gu, Hui-Kuan Lin*

*To whom correspondence should be addressed. E-mail: hklin{at}mdanderson.org

This PDF file includes:

  • Fig. S1. CYLD, but not USP7 and USP22, promotes deubiquitination of Akt.
  • Fig. S2. CYLD deubiquitinates and interacts with Akt isoforms.
  • Fig. S3. Phosphorylation of Akt does not affect its interaction with CYLD.
  • Fig. S4. Cyld–/– MEFs show enhanced basal membrane localization of Akt.
  • Fig. S5. The role of PI3K activity in the membrane recruitment of Akt in wild-type and Cyld–/– MEFs.
  • Fig. S6. CYLD deficiency enhances cell proliferation in primary MEFs and breast cancer cells.
  • Table S1. Quantification of the indicated immunoblotting signals.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.48 MB


Citation: W.-L. Yang, G. Jin, C.-F. Li, Y. S. Jeong, A. Moten, D. Xu, Z. Feng, W. Chen, Z. Cai, B. Darnay, W. Gu, H.-K. Lin, Cycles of Ubiquitination and Deubiquitination Critically Regulate Growth Factor–Mediated Activation of Akt Signaling. Sci. Signal. 6, ra3 (2013).

© 2013 American Association for the Advancement of Science