Supplementary Materials

Supplementary Materials for:

Ca2+-Dependent Phosphorylation of Ca2+ Cycling Proteins Generates Robust Rhythmic Local Ca2+ Releases in Cardiac Pacemaker Cells

Syevda Sirenko, Dongmei Yang, Yue Li, Alexey E. Lyashkov, Yevgeniya O. Lukyanenko, Edward G. Lakatta, Tatiana M. Vinogradova*

*To whom correspondence should be addressed. E-mail: vinogradovat@grc.nia.nih.gov

This PDF file includes:

  • Fig. S1. Local Ca2+ release characteristics are Ca2+-dependent and differ in sinoatrial node cells and ventricular myocytes.
  • Fig. S2. The size of spontaneous local Ca2+releases delineates their periodicity.
  • Fig. S3. High cytosolic Ca2+ concentrations eliminate local Ca2+ releases in sinoatrial node cells.
  • Fig. S4. Increased cytosolic Ca2+ concentrations increase phosphorylation of PLB at Thr17 in sinoatrial node cells, but not in ventricular myocytes.
  • Fig. S5. Increased cytosolic Ca2+ concentrations do not change the phosphorylation of PLB at Thr17 in ventricular myocytes.
  • Fig. S6. Phosphorylated RyR immunolabeling in ventricular myocytes is not Ca2+- dependent.
  • Fig. S7. Effects of PKI and AIP on the characteristics of local Ca2+ releases in permeabilized sinoatrial node cells.

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Citation: S. Sirenko, D. Yang, Y. Li, A. E. Lyashkov, Y. O. Lukyanenko, E. G. Lakatta, T. M. Vinogradova, Ca2+-Dependent Phosphorylation of Ca2+ Cycling Proteins Generates Robust Rhythmic Local Ca2+ Releases in Cardiac Pacemaker Cells. Sci. Signal. 6, ra6 (2013).

© 2013 American Association for the Advancement of Science