Supplementary Materials

Supplementary Materials for:

Dysregulated RasGRP1 Responds to Cytokine Receptor Input in T Cell Leukemogenesis

Catherine Hartzell, Olga Ksionda, Ed Lemmens, Kristen Coakley, Ming Yang, Monique Dail, Richard C. Harvey, Christopher Govern, Jeroen Bakker, Tineke L. Lenstra, Kristin Ammon, Anne Boeter, Stuart S. Winter, Mignon Loh, Kevin Shannon, Arup K. Chakraborty, Matthias Wabl, Jeroen P. Roose*

*Corresponding author. E-mail: jeroen.roose{at}ucsf.edu

This PDF file includes:

  • Fig. S1. Computational models to explore T-ALL Ras activation.
  • Fig. S2. Overview of the Rasgrp1 viral insertion map.
  • Fig. S3. Characterization of T-ALL cell lines used in this study.
  • Fig. S4. Analysis of p53 phosphorylation, p21 abundance, and the extent of apoptosis in Rasgrp1 and K-RasG12D T-ALL lines.
  • Fig. S5. In vitro proliferation of K-RasG12D and Rasgrp1 T-ALL lines.
  • Fig. S6. In vivo proliferation of K-RasG12D and Rasgrp1 T-ALL cell lines.
  • Fig. S7. Rasgrp1 abundance in 3397S-E and 1156S-O cells, and cell-survival characteristics and Rasgrp1 abundance in 1156S-O Rasgrp1 T-ALL transplants.
  • Fig. S8. PMA-induced ERK phosphorylation in K-RasG12D and Rasgrp1 T-ALL cell lines.

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Citation: C. Hartzell, O. Ksionda, E. Lemmens, K. Coakley, M. Yang, M. Dail, R. C. Harvey, C. Govern, J. Bakker, T. L. Lenstra, K. Ammon, A. Boeter, S. S. Winter, M. Loh, K. Shannon, A. K. Chakraborty, M. Wabl, J. P. Roose, Dysregulated RasGRP1 Responds to Cytokine Receptor Input in T Cell Leukemogenesis. Sci. Signal. 6, ra21 (2013).

© 2013 American Association for the Advancement of Science