Supplementary Materials

Supplementary Materials for:

Genetics and Phosphoproteomics Reveal a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis thaliana

Taishi Umezawa,* Naoyuki Sugiyama, Fuminori Takahashi, Jeffrey C. Anderson, Yasushi Ishihama, Scott C. Peck, Kazuo Shinozaki*

*Corresponding author. E-mail: taishi@cc.tuat.ac.jp (T.U.); kazuo.shinozaki@riken.jp (K.S.)

This PDF file includes:

  • Fig. S1. Experimental workflow of the phosphoproteomic analysis.
  • Fig. S2. Venn diagram of the ABA-responsive genes and dehydration-responsive genes in wild-type plants.
  • Fig. S3. Comparative analysis of ABA- or dehydration-responsive gene expression data from wild type and srk2dei.
  • Fig. S4. Motif-X analysis of the ABA- or dehydration-responsive phosphopeptides.
  • Fig. S5. In vitro kinase assay of AtMPK1 immunoprecipitated from wild-type or srk2dei.
  • Fig. S6. Expression patterns of SNS1.
  • Fig. S7. RNA gel blot analysis of ABA-responsive genes in wild type and sns1.
  • Fig. S8. SNS1 orthologs in plants.
  • Legends for Tables S1 to S3

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Microarray data from wild type and srk2dei.
  • Table S2 (Microsoft Excel format). List of phosphopeptides detected in seedlings exposed to ABA or dehydration.
  • Table S3 (Microsoft Excel format). List of ABA- or dehydration stress–responsive

    phosphopeptides.

Citation: T. Umezawa, N. Sugiyama, F. Takahashi, J. C. Anderson, Y. Ishihama, S. C. Peck, K. Shinozaki, Genetics and Phosphoproteomics Reveal a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis thaliana. Sci. Signal. 6, rs8 (2013).

© 2013 American Association for the Advancement of Science