Supplementary Materials

Supplementary Materials for:

Nutrient Signaling in Protein Homeostasis: An Increase in Quantity at the Expense of Quality

Crystal S. Conn and Shu-Bing Qian*

*Corresponding author. E-mail: sq38@cornell.edu

This PDF file includes:

  • Fig. S1. Quantification of Fluc mRNA abundance in wild-type and TSC2 knockout cells.
  • Fig. S2. Phosphorylation status of eIF2α in wild-type and TSC2 knockout cells.
  • Fig. S3. Features of GFP in wild-type and TSC2 knockout cells.
  • Fig. S4. Chaperone and proteasome activity in cells expressing Rheb.
  • Fig. S5. Quantification of mRNA abundance of Fluc mutants in wild-type and TSC2 knockout cells.
  • Fig. S6. Translation fidelity in cells expressing Rheb.
  • Fig. S7. Ratio of 28S to 18S rRNAs in wild-type and TSC2 knockout cells.
  • Fig. S8. Measurement of ribosome dynamics during translation elongation.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 750 KB


Citation: C. S. Conn, S.-B. Qian, Nutrient Signaling in Protein Homeostasis: An Increase in Quantity at the Expense of Quality. Sci. Signal. 6, ra24 (2013).

© 2013 American Association for the Advancement of Science